03 20 44 61 54
protoxyde@chu-lille.fr

Addressing the Global Challenge of Nitrous Oxide Misuse Through a Multidisciplinary Approach: Example of the PROTOSIDE Network

Guillaume Grzych1,*, Remy Diesnis2, Thierry Dupré3, Jean Paul Niguet4, Emeline Gernez5, Damien Denimal 6, Sylvie Deheul7,8, Jean Claude Guichard9, Damien Scliffet 10, Bruno Mégarbane 11, Isabelle Redonnet-Vernhet 12, Alexandra Boucher 13, Anas Bennis 14, Laurent Karila5, Etienne Cavalier16, Benjamin Rolland 17,18, Christophe Riou 17,18, Cécile Bossaert19 and Anthony Chauvin 20 on behalf of the multidisciplinary network PROTOSIDE
1 CHU Lille, Service Biochimie Automatisée-Protéines, 59000 Lille, France
2 Emergency Departement, CH Roubaix, 59100 Roubaix, France
3 Département de Biochimie, AP-HP, Hôpital Bichat, Biochimie, 75018 Paris, France
4 Service de Neurologie, Hôpital Saint Vincent de Paul-GHICL, 59000 Lille, France
5 CHU de Lille, Centre de Biologie Pathologie Génétique, 59000 Lille, France
6 CHU Dijon Bourgogne, INSERM U1231, Université Bourgogne Europe, 21000 Dijon, France
7 Inserm, UMR S1171, CHU Lille, Service de Pharmacologie Médicale, Université de Lille, 59000 Lille, France
8 CEIP-Addictovigilance, Service de Pharmacologie Médicale, CHRU de Lille, 59045 Lille, France
9 Unité Sanitaire en Milieu Pénitentiaire USMP, 59000 Lille, France
10 CH de Lens, Addictologie, 62300 Lens, France
 add Show full affiliation list
Author to whom correspondence should be addressed.
Toxics 2025, 13(6), 466; https://doi.org/10.3390/toxics13060466
Submission received: 17 April 2025 / Revised: 28 May 2025 / Accepted: 29 May 2025 / Published: 31 May 2025

Abstract

Nitrous oxide (N₂O) was originally used for medical and industrial purposes, but its recreational use has dramatically increased, raising a major global public health concern. Chronic inhalation is associated with neurological, metabolic, and psychiatric complications, as well as addiction. To address these challenges, the PROTOSIDE network was developed to provide a multidisciplinary approach to management and prevention. This initiative relies on competence centers integrating specialists in emergency medicine, neurology, clinical biochemistry, and addiction medicine. PROTOSIDE aims to standardize diagnostic protocols, optimize patient care pathways, and strengthen addictovigilance. A strong emphasis is placed on prevention, including awareness campaigns and collaboration with healthcare professionals and educators. By facilitating access to advanced biochemical analyses (homocysteine, methylmalonic acid) and promoting international guidelines, PROTOSIDE represents an innovative model for a global response to N₂O misuse. This integrated approach enhances clinical management, reduces complications, and harmonizes public health strategies.
Keywords: nitrous oxide; addiction; neurology; toxicology; international network; multidisciplinary; methylmalonic acid; homocysteine; biomarkers

Share and Cite

MDPI and ACS Style

Grzych, G.; Diesnis, R.; Dupré, T.; Niguet, J.P.; Gernez, E.; Denimal, D.; Deheul, S.; Guichard, J.C.; Scliffet, D.; Mégarbane, B.; et al. Addressing the Global Challenge of Nitrous Oxide Misuse Through a Multidisciplinary Approach: Example of the PROTOSIDE Network. 

Toxics 2025, 13, 466. https://doi.org/10.3390/toxics13060466

AMA Style
Grzych G, Diesnis R, Dupré T, Niguet JP, Gernez E, Denimal D, Deheul S, Guichard JC, Scliffet D, Mégarbane B, et al. Addressing the Global Challenge of Nitrous Oxide Misuse Through a Multidisciplinary Approach: Example of the PROTOSIDE Network.Toxics. 2025; 13(6):466. https://doi.org/10.3390/toxics13060466Chicago/Turabian Style
Grzych, Guillaume, Remy Diesnis, Thierry Dupré, Jean Paul Niguet, Emeline Gernez, Damien Denimal, Sylvie Deheul, Jean Claude Guichard, Damien Scliffet, Bruno Mégarbane, and et al. 2025. « Addressing the Global Challenge of Nitrous Oxide Misuse Through a Multidisciplinary Approach: Example of the PROTOSIDE Network »Toxics13, no. 6: 466. https://doi.org/10.3390/toxics13060466

APA Style
Grzych, G., Diesnis, R., Dupré, T., Niguet, J. P., Gernez, E., Denimal, D., Deheul, S., Guichard, J. C., Scliffet, D., Mégarbane, B., Redonnet-Vernhet, I., Boucher, A., Bennis, A., Karila, L., Cavalier, E., Rolland, B., Riou, C., Bossaert, C., & Chauvin, A., on behalf of the multidisciplinary network PROTOSIDE. (2025). Addressing the Global Challenge of Nitrous Oxide Misuse Through a Multidisciplinary Approach: Example of the PROTOSIDE Network.

Toxics,13(6), 466. https://doi.org/10.3390/toxics13060466

Tags: Actualité, Publications scientifiques

Faire un don

Adhérer à l’association !